Publications
Below, publications are listed in reverse
chronological order. After each I have started writing a short description of
the contents/significance of the paper.
46. A. Kunwar, M. Vershinin, J. Xu, and S.P. Gross,
“Stepping,
Strain Gating, and an Unexpected Force-Velocity Curve for Multiple-Motor-Based
Transport”, Current Biology 18, 1–11,
August 26, 2008 (2008). Download PDF copy of this paper Download
PDF supplement
Investigates theoretically how multiple kinesin motors function together,
and how uneven load sharing can result in enhances system performance under
load. It finds that ensemble multiple motor function depends strongly on the
coupling between the motors. It predicts—which is then confirmed
experimentally—that surprisingly, for a range of likely cytosolic
viscosities, cargos driven by a single motor can move faster than cargos moved
by two or more motors.
45. M.A. Welte and S. P. Gross, “Molecular motors: a traffic cop within?”, HFSP Journal in press,
(2008). Download PDF copy of this paper
A brief review discussing a paper from the Lipowsky group that recently appeared in PNAS. The work is very intriguing, in that it presents a model that suggests
that many aspects of bi-directional vesicular motion may be explained quantitatively
due to specific properties of single motors, and how tug-of-wars are resolved.
44. M. Vershinin, J. Xu, D. Razafsky, S.J. King, and S.P. Gross, “Tuning
microtubule-based transport through filamentous MAPs:
the problem of dynein.”
Traffic, 9(6):882-92
(2008). Download PDF copy of this paper
A previous PNAS paper (#38, below) investigated how two or three kinesin
motors function together, and showed that tau—at levels found in
cells—can function to regulate the number of engaged motors, allowing
cells the possibility to spatially regulate plus-end transport via control of
track (microtubule) accessibility. This raised the potential problem of cross-talk
between plus-end and minus-end transport, since they both occur along
microtubules. In this manuscript we investigate dynein’s
sensitivity to tau, in both the single- and multiple-motor regimes. We show
that filament-level regulation can occur without cross-talk, because dynein is essentially unaffected by the low levels to
moderate levels to tau that so significantly alter kinesin-based transport.
Using a construct (a portions of dynein’s
microtubule-binding domain), we investigate how dynein
avoids kinesins’ tau sensitivity.
43. B.C.
Carter, M. Vershinin, S.P. Gross, “A Comparison
of Step-Detection Methods: How Well Can You Do?”
Biophys. Jl, 94(1):306-19, (2008). Download PDF copy
of this paper
Investigates properties of different step-detection methods, and then
applies the best one to the problem of how multiple kinesin motors function
together. It shows that under low load, and saturating ATP, in vitro two
kinesin motors attached to a cargo do not coordinate, but instead function
independently, so that the center of mass of the cargo moves in ~4nm steps.
42. S. P. Gross, M. Vershinin
and G.T. Shubeita, “Cargo Transport: Two Motors Are Sometimes Better Than One”, Curr. Bio. v.17,
R478-486 (2007). Download PDF copy of
this paper
A review of advances in our understanding of how multiple
motors move cargos, and the ramifications of
the number of engaged motors moving cargos. Based on a summary of structural
(EM data) and in vivo force measurements, it suggests that most cargos
transported along microtubules are moved by a limited number of motors (between
1 and 5).
41. S. P. Gross, “Molecular Motors: A Tale of Two
Filaments”, Curr. Bio.
v.17, R277-280 (2007). Download PDF copy of this paper
A brief review of advances in myosin V-actin
filament-filament switching, discussing both the role of the number of motors on
the cargo, and also new results on the properties of single Myosin-V motors.
40. D.Y. Petrov, R. Mallik, G.T. Shubeita, M. Vershinin, S.P.
Gross †+, and C.C.Yu+ , “Studying Molecular Motor-based
Cargo Transport: What is Real, and What is Noise?”, Online Early
Edition, Biophys.
Jl,
(2007). Download PDF copy of this paper Download PDF
copy of this paper
+=Co-senior author †=Corresponding author
Investigates how two or three kinesin motors function together, and
shows that stall forces for motors are additive, and that multiple kinesin
motors move cargos very long distances. It then shows that tau—at levels
found in cells—can function to regulate the number of engaged motors,
allowing cells the possibility to spatially regulate transport via control of
track (microtubule) accessibility.
39. J.E.
Martinez, M.D. Vershinin , G.T. Shubeita, and
S.P. Gross, “On the use of in vivo cargo velocity as a biophysical
marker”, Biochem. Biophys. Res.
Comm. 353, 835-840 (2007). Download PDF
copy of this paper Download Word supplement Download
JPEG Fig
Investigates, both theoretically and experimentally, the
published proposal that a cargo’s velocity can be used to infer the
number of engaged motors moving the cargo. The manuscript concludes that cargo velocity is likely a poor marker
for the number of engaged motors.
38. M. Vershinin, B.C. Carter, D.S. Razafsky,
S.J. King and S.P. Gross, “Multiple-motor based transport and its
regulation by Tau”, PNAS V. 104, 87–92 (2007). (track 2) Download PDF copy of this paper Download
PDF supplement
Investigates how two or three kinesin motors function together, and
shows that stall forces for motors are additive, and that multiple kinesin
motors move cargos very long distances. It then shows that tau—at levels
found in cells—can function to regulate the number of engaged motors,
allowing cells the possibility to spatially regulate transport via control of
track (microtubule) accessibility.
37. R. Mallik and S. P. Gross, “Molecular
motors as cargo transporters in the cell —The good, the bad and the
ugly”, Physica
A, V. 372, 65 –69, (2006). Download PDF copy of this paper
A brief review of the function of molecular motors.
36. S. Cermelli*, Y. Guo,
S.P. Gross+† and M.A. Welte+,
"The Lipid-Droplet Proteome Reveals that Droplets Are a Protein-Storage
Depot", Curr. Bio., v.
16, 1783-1795, (2006). Download PDF copy
of this paper Download PDF supplement
+=Co-senior author †=Corresponding
author
Uses mass spectrometry to determine the proteins present on embryonic
lipid droplets. Because the proteins
present are found to be highly conserved between Drosophila and mammalian
droplets, this suggests that studying the Drosophila droplets can provide
important insights into the similar processes in mammals. In the proteome,
certain unexpected proteins—histones—are
present in large amounts The localization of histones to the droplets is investigated in depth, and
concluded to be real, and temporally regulated. Based on these findings,
together with published observations from others, the manuscript proposes a new
model for lipid droplets as generalized sites of protein storage/sequestration.
35. S. L. Bullock, A. Nicol, S.P. Gross,
and D. Zicha, " Guidance of Bidirectional Motor Complexes by mRNA Cargoes through
Control of Dynein Number and Activity", Curr. Bio.,V. 16, 1447–1452, (2006). Download PDF copy of
this paper
34. S.E. Antinone,
G.T. Shubeita, K.E. Coller,
J.I. Lee, S. Haverlock-Moyns, S.P. Gross+,
and G.A. Smith+, "The herpesvirus
capsid surface protein, VP26, and the majority of the
tegument proteins are dispensable for capsid
transport toward the nucleus", J. Virol.,
n. 80, 5494–5498 (2006). Download PDF copy of
this paper
33. Roop Mallik, Dmitri Petrov, S.A. Lex, S.J. King, and S.P.
Gross, "Building Complexity: An In Vitro Study of Cytoplasmic Dynein with In Vivo Implications", Curr.
Bio., v. 15, 2075-2085, (2005). Download PDF copy of this paper Download PDF supplement
Investigates how two or three dynein motors function together, and shows that stall
forces for motors are additive, and that multiple dynein
motors move cargos very long distances. Shows that a cargo moved by two dyneins is expected to move a very long distance, so that
for cargos moved by two or more dynein motors in
cells, the dynactin complex (which increases dynein processivity) is likely
unnecessary as far as facilitating travel distance. This does not mean, however,
that the dynactin complex is unimportant—we
have previously shown that in some cases it plays a role in coordinating
kinesin and dynein, and others have shown that it
frequently plays an important role in dynein-cargo
attachment.
32. M.P. Singh, R. Mallik, S. P. Gross § , and C.C.
Yu § , "
31. M.A. Welte*,
S. Cermelli*, J. Griner, A.
Viera, Y. Guo, D. Kim, J.G.
Gindhart , S.P. Gross, "Regulation
of lipid-droplet transport by the Perilipin homologue
LSD2", Curr. Bio., v.
15, 1266-1275, (2005). Download PDF copy of this paper Download PDF supplement
30. F. Lin, CM Nguyen,
SJ Wang, W Saadi, SP Gross, NL Jeon, "Neutrophil
Migration in Opposing Chemoattractant Gradients Using
Microfluidic Chemotaxis
Devices", Ann. Biom. Engin., v. 33, no. 4,
475-482 (2005). Download PDF copy of
this paper
29. Brian C. Carter, George T. Shubeita, and Steven P. Gross, “ Tracking
single-particles: a user-friendly quantitative
evaluation", Physical Biology 2, 60–72, (2005). Download PDF copy of this paper
28. T. del
27. Roop Mallik and Steven P. Gross, “Molecular Motors:
Strategies to Get Along”, Current Biology, v. 14, R971-R982,
(2004). Download PDF copy of this paper
26. G.A. Smith, L. Pomeranz, S.P. Gross§ and L.
Enquist§, “Local modulation of plus-end
transport targets herpesvirus entry and egress in
sensory axons", PNAS early eddition, (2004) (§
=co-senior author) Download PDF copy of this paper
25. J. Snider, F. Lin,
24. SP Gross “Hither and yon:
a review of bi-directional microtubule-based transport", Physical Biology
1: R1–R11, (2004) Download PDF copy of this paper
23. F. Lin, CM Nguyen, SJ
Wang,
22. R. Mallik,
B.C. Carter, S.A. Lex, S.J. King and S.P. Gross
“Cytoplasmic dynein functions as a gear in
response to load”, Nature 427, 649-52 (2004). Download
PDF copy of this paper
21. Vladimir Rodionov,
Julie Yi, Anna Kashina, Abiola
Oladipo, and Steven P. Gross, “Switching
between microtubule- and actin-based transport
systems in melanophores is controlled by cAMP levels”, Current Biology, v. 13,
1837–1847, (2003). Download PDF copy of
this paper
20. Steven P. Gross,Yi
Guo, Joel E. Martinez, and Michael A. Welte, “A Determinant for Directionality of
Organelle Transport in Drosophila Embryos”, Current Biology, v. 13,
1660–1668, (2003). Download PDF copy of this
paper
19. S. P. Gross, “Dynactin: Coordinating Motors with Opposite Inclinations
(Dispatch)”, Current Biology, v. 13, R320-322 (2003). Download PDF copy of this paper
18. S. P. Gross, “Application
of Optical Traps In Vivo”, Methods in Enzymology,
v. 361, 162-174 (2003). Download PDF copy
of this paper
17. L.J. Davis, D.J. Odde, S. M. Block, and S. P. Gross, “The Importance
of Lattice Defects in Katanin-Mediated Microtubule
Severing in Vitro”, Biophys. J. 82, 2916-27
(2002). Download PDF copy of this paper
16. S. P. Gross*, M. C. Tuma*, S. W. Deacon, A. S. Serpinskaya
A. R. Reilein and V.
15. S.P Gross*, M. Welte*, S.M. Block, and E.F. Wieschaus,
“Coordination of opposite-polarity microtubule motors”, J. Cell
Bio. 156, 715-24, (2002) Download
PDF copy of this paper
14. L.W. Enquist,
M.J. Tomishima, S. Gross, G.S. Smith,
“Directional spread of an alpha-herpsesvirus in
the nervous system”, Veter. Microb. 2266, 1-12
(2002). Download PDF copy of this paper
13. G.A. Smith*,
S.P Gross*, and L.W. Enquist, “Herpesviruses use bidirectional fast-axonal transport to
spread in sensory neurons”, PNAS 98 3466-70 (2001) Download
PDF copy of this paper
12. S.P Gross, M. Welte, S.M. Block, and E.F. Wieschaus,
“Dynein-mediated cargo transport In vivo. A
switch controls travel distance.”, J. Cell Biol. 148 945-56 (2000). Download PDF copy of this paper
11. S.P. Gross*, M. Welte*, M. Postner, S. M. Block,
and E.F. Wieschaus, “Developmental and Genetic
Regulation of Vesicle Transport in Drosphila
Embryos”, Cell 92, 547 (1998). Download PDF copy of
this paper
10. K. Visscher,
S. P. Gross, and S. M. Block, “Construction of Multiple-Beam Optical
Traps with Nanometer-Resolution Position Sensing”, IEEE Jl. Sel. Top.
Quant. Electr., 2, 1066 (1996). Download PDF copy of this paper
9. E. Sharon, S. P. Gross, and J. Fineberg, “Energy Dissipation in Dynamic
Fracture,” Phys. Rev. Lett., 76, 2117 (1996).
8. S.P. Gross, “Instabilities
in Fast Fracture”, Ph.D. Dissertation,
7.
6. M. Marder
and Steve Gross, ``Origin of Crack Tip Instabilities,'' Jl. of the Mech. and
Phys. of Sol., 43, 1 (1995).
5. S. P. Gross, J. Fineberg, M. Marder, W.D.
McCormick, and H. L. Swinney, ``Acoustic Emissions
from Rapidly Moving Cracks," Phys. Rev. Lett.,
71, 3162 (1993).
4. J. Fineberg,
S. P. Gross, M. Marder, and H. L. Swinney,
``Instability in the propagation of fast cracks'', Phys. Rev. B, 45, 5146,
1992.
3. J. Fineberg,
S. P. Gross, M. Marder, and H. L. Swinney,
``Instability in Dynamic Fracture,'' Phys. Rev. Lett.,
67, 457, (1991).
2. S. Gross, G. Zocchi,
and A. Libchaber ``Waves and Plumes of thermal
boundary layer,'' C. R. Acad. Sci. Paris, 307, Serie
II, 447, (1988).
1. J. Glazier, S. P. Gross, J. Stavans, ``Dynamics of two-dimensional soap froths'', Phys.
Rev. A, 36, 306, 1987.
*= Joint first authors.
