Publications
Below, publications are listed in
reverse chronological order. After each I have started writing a short
description of the contents/significance of the paper.
51. M. Ingelmo-Torres,
E. González-Moreno, A. Kassan,
M. Hanzal-Bayer, F. Tebar, A.
Herms, T. Grewal, J.F. Hancock, C. Enrich, M. Bosch,
S.P. Gross, R.G. Parton and A. Pol, “Hydrophobic
and Basic Domains Target Proteins to Lipid Droplets”, Traffic, in press (2009) Download PDF copy of this paper
Investigates how proteins such as Caveolin
are targeted to Lipid Droplets.
50. R. Mallik
and SP Gross, “Intracellular transport: how do motors work together?”, Curr Biol.19(10), (2009) Download
PDF copy of this paper
A short dispatch on interesting work
investigating transport of intra-flagellar particles.
49. F. Ziebert,
M. Vershinin, SP Gross, IS Aranson,
“Collective alignment of polar filaments by molecular motors.” Eur
Phys J E Soft Matter, 28(4), (2009) Download
PDF copy of this paper
Investigates theoretically and experimentally how
molecular motors can align filaments by cross-linking and moving.
48. G. Shubeita,
S. Tran, J. Xu, M. Vershinin,
Establishes that for lipid droplets, simply
controlling the overall number of motors does not result in changes to droplet
motion.
47. K. Larsen, J. Xu,
Establishes that BicD plays a
role in regulating lipid droplet motion in drosophila embryos, and shows that BicD plays a dynamic rather than static role in controlling
such motion.
46. A. Kunwar,
M. Vershinin, J. Xu, and
S.P. Gross, “Stepping, Strain Gating, and an Unexpected Force-Velocity Curve for
Multiple-Motor-Based Transport”,
Current Biology 18, 1–11, August 26, 2008 (2008). Download PDF copy of
this paper Download PDF supplement
Investigates theoretically how multiple kinesin motors
function together, and how uneven load sharing can result in enhances system
performance under load. It finds that ensemble multiple motor function depends
strongly on the coupling between the motors. It predicts—which is then
confirmed experimentally—that surprisingly, for a range of likely cytosolic
viscosities, cargos driven by a single motor can move faster than cargos moved
by two or more motors.
45. M.A. Welte
and S. P. Gross, “Molecular motors: a traffic cop within?”, HFSP Journal in press, (2008). Download PDF copy
of this paper
A brief review discussing a paper from the Lipowsky group that recently appeared in PNAS. The work is very intriguing, in that it presents a model
that suggests that many aspects of bi-directional vesicular motion may be
explained quantitatively due to specific properties of single motors, and how
tug-of-wars are resolved.
44. M. Vershinin, J. Xu, D. Razafsky, S.J. King, and
S.P. Gross, “Tuning microtubule-based transport through filamentous MAPs: the
problem of dynein.”
Traffic, 9(6):882-92 (2008). Download PDF
copy of this paper
A previous PNAS paper (#38, below) investigated how two
or three kinesin motors function together, and showed that tau—at levels found
in cells—can function to regulate the number of engaged motors, allowing cells
the possibility to spatially regulate plus-end transport via control of track
(microtubule) accessibility. This raised the potential problem of cross-talk
between plus-end and minus-end transport, since they both occur along microtubules.
In this manuscript we investigate dynein’s
sensitivity to tau, in both the single- and multiple-motor regimes. We show
that filament-level regulation can occur without cross-talk, because dynein is essentially unaffected by the low levels to
moderate levels to tau that so significantly alter kinesin-based transport.
Using a construct (a portions of dynein’s
microtubule-binding domain), we investigate how dynein
avoids kinesins’ tau sensitivity.
43. B.C. Carter, M. Vershinin,
S.P. Gross, “A Comparison of Step-Detection Methods: How Well Can You Do?”
Biophys. Jl,
94(1):306-19, (2008). Download
PDF copy of this paper
Investigates properties of different step-detection
methods, and then applies the best one to the problem of how multiple kinesin
motors function together. It shows that under low load, and saturating ATP, in
vitro two kinesin motors attached to a cargo do not coordinate, but instead
function independently, so that the center of mass of the cargo moves in ~4nm
steps.
42. S. P. Gross, M. Vershinin
and G.T. Shubeita, “Cargo Transport: Two Motors Are Sometimes
Better Than One”, Curr. Bio.
v.17, R478-486 (2007). Download PDF copy of this paper
A review of advances in our understanding of
how multiple motors move cargos, and the
ramifications of the number of engaged motors moving cargos. Based on a summary
of structural (EM data) and in vivo force measurements, it suggests that most
cargos transported along microtubules are moved by a limited number of motors
(between 1 and 5).
41. S. P. Gross, “Molecular Motors: A Tale of Two
Filaments”, Curr. Bio. v.17, R277-280 (2007). Download
PDF copy of this paper
A brief review of advances in myosin V-actin filament-filament switching, discussing both the role
of the number of motors on the cargo, and also new results on the properties of
single Myosin-V motors.
40. D.Y. Petrov, R. Mallik, G.T. Shubeita, M. Vershinin, S.P. Gross †+, and C.C.Yu+ ,
“Studying Molecular Motor-based Cargo Transport: What is Real, and What is
Noise?”, Online Early Edition, Biophys. Jl, (2007). Download PDF copy of this
paper Download
PDF copy of this paper
+=Co-senior author †=Corresponding
author
Investigates how two or three kinesin motors function
together, and shows that stall forces for motors are additive, and that
multiple kinesin motors move cargos very long distances. It then shows that
tau—at levels found in cells—can function to regulate the number of engaged
motors, allowing cells the possibility to spatially regulate transport via
control of track (microtubule) accessibility.
39. J.E. Martinez, M.D. Vershinin , G.T. Shubeita, and S.P. Gross, “On the use of in vivo cargo
velocity as a biophysical marker”, Biochem. Biophys. Res. Comm. 353, 835-840 (2007). Download PDF copy of this paper Download Word supplement
Download
JPEG Fig
Investigates, both theoretically and
experimentally, the published proposal that a cargo’s velocity can be used to
infer the number of engaged motors moving the cargo. The manuscript concludes that cargo velocity is likely a
poor marker for the number of engaged motors.
38. M. Vershinin, B.C. Carter,
D.S. Razafsky, S.J. King and S.P. Gross,
“Multiple-motor based transport and its regulation by Tau”, PNAS V. 104, 87–92
(2007). (track 2) Download PDF copy of
this paper Download PDF supplement
Investigates how two or three kinesin motors function together,
and shows that stall forces for motors are additive, and that multiple kinesin
motors move cargos very long distances. It then shows that tau—at levels found
in cells—can function to regulate the number of engaged motors, allowing cells
the possibility to spatially regulate transport via control of track
(microtubule) accessibility.
37. R. Mallik
and S. P. Gross, “Molecular
motors as cargo transporters in the cell —The good, the bad and the ugly”, Physica A, V.
372, 65 –69, (2006). Download PDF copy of this paper
A brief review of the function of molecular
motors.
36. S. Cermelli*,
Y. Guo, S.P. Gross+†
and M.A. Welte+,
"The Lipid-Droplet Proteome Reveals that Droplets Are a Protein-Storage
Depot", Curr. Bio., v.
16, 1783-1795, (2006). Download PDF copy
of this paper Download PDF supplement
+=Co-senior
author †=Corresponding author
Uses mass spectrometry to determine the proteins present
on embryonic lipid droplets. Because the
proteins present are found to be highly conserved between Drosophila and
mammalian droplets, this suggests that studying the Drosophila droplets can
provide important insights into the similar processes in mammals. In the
proteome, certain unexpected proteins—histones—are
present in large amounts The localization of histones to the droplets is investigated in depth, and
concluded to be real, and temporally regulated. Based on these findings,
together with published observations from others, the manuscript proposes a new
model for lipid droplets as generalized sites of protein
storage/sequestration.
35.
S. L. Bullock, A. Nicol, S.P. Gross, and D. Zicha, " Guidance of Bidirectional
Motor Complexes by mRNA Cargoes through Control of Dynein
Number and Activity",
Curr. Bio.,V.
16,
1447–1452, (2006). Download PDF copy of this paper
34. S.E. Antinone,
G.T. Shubeita, K.E. Coller,
J.I. Lee, S. Haverlock-Moyns, S.P. Gross+,
and G.A. Smith+, "The herpesvirus
capsid surface protein, VP26, and the majority of the
tegument proteins are dispensable for capsid
transport toward the nucleus", J. Virol.,
n. 80, 5494–5498 (2006). Download PDF copy of this
paper
33. Roop Mallik, Dmitri Petrov, S.A. Lex, S.J. King, and S.P.
Gross, "Building Complexity: An In Vitro Study of Cytoplasmic
Dynein with In Vivo Implications", Curr. Bio., v. 15, 2075-2085,
(2005). Download PDF copy of this paper
Download PDF supplement
Investigates how two
or three dynein motors function together, and shows
that stall forces for motors are additive, and that multiple dynein motors move cargos very long distances. Shows that a
cargo moved by two dyneins is expected to move a very
long distance, so that for cargos moved by two or more dynein
motors in cells, the dynactin complex (which
increases dynein processivity)
is likely unnecessary as far as facilitating travel distance. This does not mean, however,
that the dynactin complex is unimportant—we have
previously shown that in some cases it plays a role in coordinating kinesin and dynein, and others
have shown that it frequently plays an important role in dynein-cargo
attachment.
32. M.P. Singh, R. Mallik, S. P. Gross § , and C.C.
Yu § , "
31. M.A. Welte*,
S. Cermelli*, J. Griner, A.
Viera, Y. Guo, D. Kim, J.G.
Gindhart , S.P. Gross, "Regulation
of lipid-droplet transport by the Perilipin homologue
LSD2", Curr. Bio., v.
15, 1266-1275, (2005). Download PDF copy of this paper Download PDF supplement
30. F. Lin, CM Nguyen,
SJ Wang, W Saadi, SP Gross, NL Jeon, "Neutrophil
Migration in Opposing Chemoattractant Gradients Using
Microfluidic Chemotaxis
Devices", Ann. Biom. Engin., v. 33, no. 4,
475-482 (2005). Download PDF copy of
this paper
29. Brian C. Carter, George T. Shubeita, and Steven P. Gross, “ Tracking
single-particles: a user-friendly quantitative
evaluation", Physical Biology 2, 60–72, (2005). Download PDF copy of this paper
28. T. del
27. Roop Mallik and Steven P. Gross, “Molecular Motors:
Strategies to Get Along”, Current Biology, v. 14, R971-R982, (2004). Download PDF copy of this paper
26. G.A. Smith, L. Pomeranz, S.P. Gross§ and L.
Enquist§, “Local modulation of plus-end
transport targets herpesvirus entry and egress in
sensory axons", PNAS early eddition, (2004) (§
=co-senior author) Download PDF copy of this paper
25. J. Snider, F. Lin,
24. SP Gross “Hither and yon: a
review of bi-directional microtubule-based transport", Physical Biology 1:
R1–R11, (2004) Download PDF copy of this paper
23. F. Lin, CM Nguyen, SJ
Wang,
22. R. Mallik,
B.C. Carter, S.A. Lex, S.J. King and S.P. Gross “Cytoplasmic dynein functions as a
gear in response to load”, Nature 427, 649-52 (2004). Download
PDF copy of this paper
21. Vladimir Rodionov,
Julie Yi, Anna Kashina, Abiola
Oladipo, and Steven P. Gross, “Switching
between microtubule- and actin-based transport
systems in melanophores is controlled by cAMP levels”, Current Biology, v. 13, 1837–1847,
(2003). Download PDF copy of this paper
20. Steven P. Gross,Yi
Guo, Joel E. Martinez, and Michael A. Welte, “A Determinant for Directionality of Organelle
Transport in Drosophila Embryos”, Current Biology, v. 13, 1660–1668,
(2003). Download PDF copy of this paper
19. S. P. Gross, “Dynactin: Coordinating Motors with Opposite Inclinations
(Dispatch)”, Current Biology, v. 13, R320-322 (2003). Download PDF copy of this paper
18. S. P. Gross, “Application of
Optical Traps In Vivo”, Methods in Enzymology, v.
361, 162-174 (2003). Download PDF copy of
this paper
17. L.J. Davis, D.J. Odde, S. M. Block, and S. P. Gross, “The Importance of
Lattice Defects in Katanin-Mediated Microtubule
Severing in Vitro”, Biophys. J. 82, 2916-27 (2002). Download PDF copy of this paper
16. S. P. Gross*, M. C. Tuma*, S. W. Deacon, A. S. Serpinskaya
A. R. Reilein and V.
15. S.P Gross*, M. Welte*, S.M. Block, and E.F. Wieschaus,
“Coordination of opposite-polarity microtubule motors”, J. Cell Bio. 156, 715-24, (2002) Download
PDF copy of this paper
14. L.W. Enquist,
M.J. Tomishima, S. Gross, G.S. Smith, “Directional
spread of an alpha-herpsesvirus in the nervous
system”, Veter. Microb. 2266, 1-12 (2002). Download PDF copy of this paper
13. G.A. Smith*,
S.P Gross*, and L.W. Enquist, “Herpesviruses
use bidirectional fast-axonal transport to spread in sensory neurons”, PNAS 98
3466-70 (2001) Download PDF copy of this paper
12. S.P Gross, M. Welte, S.M. Block, and E.F. Wieschaus,
“Dynein-mediated cargo transport In vivo. A switch
controls travel distance.”, J. Cell Biol. 148 945-56 (2000). Download PDF copy of this paper
11. S.P. Gross*, M. Welte*, M. Postner, S. M. Block,
and E.F. Wieschaus, “Developmental and Genetic
Regulation of Vesicle Transport in Drosphila
Embryos”, Cell 92, 547 (1998). Download PDF copy of this
paper
10. K. Visscher,
S. P. Gross, and S. M. Block, “Construction of Multiple-Beam Optical Traps with
Nanometer-Resolution Position Sensing”, IEEE Jl. Sel. Top. Quant. Electr., 2, 1066 (1996). Download
PDF copy of this paper
9. E. Sharon, S. P. Gross, and J. Fineberg, “Energy Dissipation in Dynamic Fracture,” Phys.
Rev. Lett., 76, 2117 (1996).
8. S.P. Gross, “Instabilities in
Fast Fracture”, Ph.D. Dissertation,
7.
6. M. Marder
and Steve Gross, ``Origin of Crack Tip Instabilities,'' Jl. of the Mech. and
Phys. of Sol., 43, 1 (1995).
5. S. P. Gross, J. Fineberg, M. Marder, W.D.
McCormick, and H. L. Swinney, ``Acoustic Emissions
from Rapidly Moving Cracks," Phys. Rev. Lett.,
71, 3162 (1993).
4. J. Fineberg,
S. P. Gross, M. Marder, and H. L. Swinney,
``Instability in the propagation of fast cracks'', Phys. Rev. B, 45, 5146,
1992.
3. J. Fineberg,
S. P. Gross, M. Marder, and H. L. Swinney,
``Instability in Dynamic Fracture,'' Phys. Rev. Lett.,
67, 457, (1991).
2. S. Gross, G. Zocchi,
and A. Libchaber ``Waves and Plumes of thermal
boundary layer,'' C. R. Acad. Sci. Paris, 307, Serie
II, 447, (1988).
1. J. Glazier, S. P. Gross, J. Stavans, ``Dynamics of two-dimensional soap froths'', Phys.
Rev. A, 36, 306, 1987.
*= Joint first authors.
